Application

Research Sub CategoryNuclear Receptors

Western Blotting Analysis: A representative lot detected ATRX in nuclear extracts from EBV-transformed lymphocytes (McDowell, T.L., et al. (1999). PNAS. 96(24):13983-13988).
Immunocytochemistry Analysis: A representative lot detected nuclear localization of ATRX in HeLa and L929 cells (McDowell, T.L., et al. (1999). PNAS. 96(24):13983-13988).

Research CategoryEpigenetics & Nuclear Function

Anti-ATRX Antibody, clone 23C is an antibody against ATRX for use in Western Blotting and Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Transcriptional regulator ATRX (EC 3.6.4.12; UniProt P46100; also known as Alpha thalassemia/mental retardation syndrome X-linked, ATP-dependent helicase ATRX, DNA dependent ATPase and helicase, Helicase 2 X-linked, X-linked helicase II, X-linked nuclear protein, XNP, Zinc finger helicase, Znf-HX) is encoded by the PRF1 (Also known as ATR2, JMS, MRXHF1, RAD54, RAD54L, SFM1, SHS, XH2, XNP, ZNF-HX) gene in human (Gene ID 546). Rad54 belongs to the SWI/SNF family of chromatin remodeling proteins. Rad54 undergoes cell cycle-dependent phosphorylation, nuclear matrix and chromatin association, and is implicatetd in playing a role in gene regulation during interphase and mitosis. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome. most often accompanied by alpha-thalassemia syndrome, as a result of diverse changes in DNA methylation.

Immunogen

Recombinant protein corresponding to the N-terminus of human ATRX.

Epitope: N-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATRX in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~283 kDa observed